Knowledge-enriched characterization of cell populations. Morphological information from pictures can include towards the richness of flow cytometry knowledge. For instance, the figure reveals an in any other case conventional apoptosis assay using Annexin V and PI, including mobile imaging to characterize cells in Each individual populace to expose morphologically distinctive features. These insights could not have been attained from multiplex staining by itself.
As samples are acquired within the Attune CytPix Flow Cytometer, the substantial-velocity brightfield digicam captures and shops illustrations or photos of detected gatherings in a charge around 6,000 visuals for every second, depending on the flow price and picture size. For increased flexibility, Attune Cytometric Software lets you regulate the impression seize frequency as wanted and select a particular gate for image seize, if demanded.
Blend acoustic concentrating technologies with large-parameter Assessment for versatile spectral unmixing and standard workflows.
Discover new insights into your experimental information by visualizing populations using put together fluorescence and morphology facts. Our program interface wizards allow downsampling, concatenation (merged documents) and dimensionality equipment which have been built in the software package for an intuitive, streamlined info Assessment working experience.
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Here, a qualified consumer has gated singlets confidently. Soon after analyzing the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate has in excess of 4% aggregates.
Optimizing gating system. Even robust manual singlet gating is mistake-inclined and stays a subjective choice stage in Pretty much all flow cytometry assays. Imaging can be employed to verify and change gates to incorporate only one cells of curiosity.
Consistent impression good quality even at large throughput Acoustic focusing and also a significant-velocity digicam combine to graphic these T cells continually at very low or high flow rates. Conveniently adjust aim and digicam configurations to fulfill experimental specifications.
The Attune NxT cytometer may be the trustworthy workhorse instrument your lab can depend on for reliable and dependable functionality. The Attune CytPix design encompasses a high-speed brightfield camera and AI-driven impression Investigation software, with the latest upgrades which includes equipment Finding out resources and an intuitive person interface, enabling enhanced information precision, precision, and a lot quicker insights into sample biology.
Buyers can see celebration images in authentic-time to verify sample high quality, optimize protocols, and history the morphology of each and every mobile population determined for Evaluation. Imaging seize also Added benefits from acoustic focusing, which helps to posture the cells in order that a pointy, centered picture is received.
Sound waves permit precise mobile alignment, resulting in exceptional info good quality and ~10X speedier sample flow fees.
We Formerly shown the strength of imaging Auto-T/Ramos cell interactions. Permit’s have a look at just the inhabitants of finest desire, the double positive gatherings, To find out more. We can easily now use prolonged morphological parameters (below, circularity and skewness of intensity) to even further analyze the options of such populations and refine gating on these activities, expanding info robustness.
Leverage the power of advanced technological know-how and innovation to speed up your discoveries. Have faith in during the Attune Flow Cytometer household to deliver the reliability, speed, and accuracy that set your perform apart.
Versatile payment prepare from Agilent Monetary Alternatives let you have the crucial instrumentation you need for quick undertaking, or to increase your lab.
Gating on these prolonged parameters helps you to promptly and accurately detect populations of fascination and ensure gating approach with little if any handbook review. The Attune program provides entry to state-of-the-art equipment Discovering analysis equipment inside an expertly intended user interface, rendering it exceptionally easy to use.
The Attune Xenith cytometer is a substantial-parameter spectral analyzer, offering both of those spectral unmixing and traditional flow capabilities for detailed and versatile mobile Assessment.
Agilent Benefit Guarantee ensures you at the very least a decade of instrument use website from the date you buy. In any other case, We're going to credit rating you Together with the residual value of the procedure towards an upgraded model.
Listed here, we clearly show that by gating tactics applying image-based quantitative parameters can distinguish interacting cells from coincident functions to far more accurately assess interacting cells.